Modified Procedure of Starch Gel Electrophoresis for β-Casein Phenotyping

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals.

Experience with the method of zone electrophoresis in starch gels (Smithies, 1955) suggests that the resolution of protein components is lessened by the use of supporting substances (e.g. filter paper or starch grains) to prevent electro-decantation from occurring during the entry of migrating proteins into the gels. The present paper describes a simple procedure which enables the sample to be ...

Caeruloplasmin as demonstrated by starch gel electrophoresis.

Starch gel electrophoresis has demonstrated that graded concentrations of sodium sulphate do not precipitate the serum globulins in any reasonable degree of purity. The use of this form of electrophoresis has shown that caeruloplasmin is associated with a globulin in the Fa(2) position.This technique can be employed as a screening test for patients suspected of suffering from hepatolenticular d...

A modified method for protein separation by zone electrophoresis on a starch gel.

In the present paper (1) a method of preparative zone electrophoresis (cf. 2, 3) is described in which a stiff starch gel with only 7 to 8 per cent of solid constituents is used as a supporting medium. Two simple devices will be described, one for the fractionation of 2 ml. of protein solution and the other for processing up to 50 ml. of solution, containing 5 to 10 per cent of protein mixture....